Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.
Identifieur interne : 000797 ( Main/Exploration ); précédent : 000796; suivant : 000798Risk factors and characteristics of low pathogenic avian influenza virus isolated from commercial poultry in Tunisia.
Auteurs : Wafa Tombari [Tunisie] ; Mathilde Paul [France] ; Jihene Bettaieb [Tunisie] ; Imen Larbi [Tunisie] ; Jihene Nsiri [Tunisie] ; Imen Elbehi [Tunisie] ; Latifa Gribaa [Tunisie] ; Abdeljelil Ghram [Tunisie]Source :
- PLoS ONE [ 1932-6203 ] ; 2013.
Abstract
OBJECTIVE: Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS: This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of specific influenza virus antibodies using cELISA test. Additionally, swab samples were tested by real time and conventional RT-PCR and compared with results obtained by others assays. Phylogenetic and genetic analyses of the glycoproteins were established for some strains. RESULTS: Out of the 800 chicken and turkey flocks tested by cELISA, 223 showed positive anti-NP antibodies (28.7%, 95% CI: 25.6-32.1). Significantly higher seroprevalence was found among the coastal areas compared to inland and during the autumn and winter. Broiler flocks showed significantly lower seroprevalence than layers and broiler breeders. The influenza virus infection prevalence increased after the laying phase among layer flocks. In addition, AIV seropositivity was significantly associated with low biosecurity measures. The Ag EIA and rRT-PCR tests revealed significantly higher numbers of AI positive samples as compared to cell cultures or egg inoculation. All new strains were subtyped as H9N2 by real time and conventional RT-PCR. Drift mutations, addition or deletion of glycosylation sites were likely to have occurred in the HA and NA glycoproteins of Tunisian strains resulting in multiple new amino acid substitutions. This fact may reflect different evolutionary pressures affecting these glycoproteins. The role of these newly detected substitutions should be tested. CONCLUSION: Our findings highlight the potential risk of AIV to avian health. Strict enforcement of biosecurity measures and possible vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV prevalence and avoid emergence of more pathogenic strains.
Url:
DOI: 10.1371/journal.pone.0053524
Affiliations:
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Le document en format XML
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</org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Elbehi, Imen" sort="Elbehi, Imen" uniqKey="Elbehi I" first="Imen" last="Elbehi">Imen Elbehi</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Gribaa, Latifa" sort="Gribaa, Latifa" uniqKey="Gribaa L" first="Latifa" last="Gribaa">Latifa Gribaa</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-409923" status="VALID"> <orgName>Laboratoire d’Epidémiologie et de Microbiologie Vétérinaire</orgName> <orgName type="acronym">LR11IPT03</orgName> <desc> <address> <addrLine>Tunis</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/index.php?option=com_content&view=article&id=364:epidemiologie-et-de-microbiologie-veterinaire&catid=91&Itemid=716</ref> </desc> <listRelation> <relation active="#struct-300103" type="direct"></relation> <relation active="#struct-301247" type="indirect"></relation> </listRelation> <tutelles><tutelle active="#struct-300103" type="direct"><org type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> </org></tutelle><tutelle active="#struct-301247" type="indirect"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author><author><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name><affiliation wicri:level="1"><hal:affiliation type="institution" xml:id="struct-300103" status="VALID"> <orgName>Institut Pasteur de Tunis</orgName> <desc> <address> <addrLine>13, place Pasteur, BP 74, 1002 Tunis Belvédère</addrLine> <country key="TN"></country> </address> <ref type="url">http://www.pasteur.tn/</ref> </desc> <listRelation> <relation active="#struct-301247" type="direct"></relation> </listRelation> <tutelles><tutelle active="#struct-301247" type="direct"><org type="regroupinstitution" xml:id="struct-301247" status="VALID"> <orgName>Réseau International des Instituts Pasteur</orgName> <orgName type="acronym">RIIP</orgName> <desc> <address> <addrLine>25, rue du Dr Roux 75724 Paris Cedex 15</addrLine> <country key="FR"></country> </address> <ref type="url">https://research.pasteur.fr/ip-network</ref> </desc> </org></tutelle></tutelles></hal:affiliation><country>Tunisie</country></affiliation></author></analytic><idno type="DOI">10.1371/journal.pone.0053524</idno><series><title level="j">PLoS ONE</title><idno type="ISSN">1932-6203</idno><imprint><date type="datePub">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"> <p>OBJECTIVE: Estimate the seroprevalence of influenza A virus in various commercial poultry farms and evaluate specific risk factors as well as analyze their genetic nature using molecular assays. MATERIALS AND METHODS: This report summarizes the findings of a national survey realized from October 2010 to May 2011 on 800 flocks in 20 governorates. Serum samples were screened for the presence of specific influenza virus antibodies using cELISA test. Additionally, swab samples were tested by real time and conventional RT-PCR and compared with results obtained by others assays. Phylogenetic and genetic analyses of the glycoproteins were established for some strains. RESULTS: Out of the 800 chicken and turkey flocks tested by cELISA, 223 showed positive anti-NP antibodies (28.7%, 95% CI: 25.6-32.1). Significantly higher seroprevalence was found among the coastal areas compared to inland and during the autumn and winter. Broiler flocks showed significantly lower seroprevalence than layers and broiler breeders. The influenza virus infection prevalence increased after the laying phase among layer flocks. In addition, AIV seropositivity was significantly associated with low biosecurity measures. The Ag EIA and rRT-PCR tests revealed significantly higher numbers of AI positive samples as compared to cell cultures or egg inoculation. All new strains were subtyped as H9N2 by real time and conventional RT-PCR. Drift mutations, addition or deletion of glycosylation sites were likely to have occurred in the HA and NA glycoproteins of Tunisian strains resulting in multiple new amino acid substitutions. This fact may reflect different evolutionary pressures affecting these glycoproteins. The role of these newly detected substitutions should be tested. CONCLUSION: Our findings highlight the potential risk of AIV to avian health. Strict enforcement of biosecurity measures and possible vaccination of all poultry flocks with continuous monitoring of poultry stations may ensure reduction of AIV prevalence and avoid emergence of more pathogenic strains.</p> </div></front></TEI><affiliations><list><country><li>France</li><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Tombari, Wafa" sort="Tombari, Wafa" uniqKey="Tombari W" first="Wafa" last="Tombari">Wafa Tombari</name></noRegion><name sortKey="Bettaieb, Jihene" sort="Bettaieb, Jihene" uniqKey="Bettaieb J" first="Jihene" last="Bettaieb">Jihene Bettaieb</name><name sortKey="Elbehi, Imen" sort="Elbehi, Imen" uniqKey="Elbehi I" first="Imen" last="Elbehi">Imen Elbehi</name><name sortKey="Ghram, Abdeljelil" sort="Ghram, Abdeljelil" uniqKey="Ghram A" first="Abdeljelil" last="Ghram">Abdeljelil Ghram</name><name sortKey="Gribaa, Latifa" sort="Gribaa, Latifa" uniqKey="Gribaa L" first="Latifa" last="Gribaa">Latifa Gribaa</name><name sortKey="Larbi, Imen" sort="Larbi, Imen" uniqKey="Larbi I" first="Imen" last="Larbi">Imen Larbi</name><name sortKey="Nsiri, Jihene" sort="Nsiri, Jihene" uniqKey="Nsiri J" first="Jihene" last="Nsiri">Jihene Nsiri</name></country><country name="France"><noRegion><name sortKey="Paul, Mathilde" sort="Paul, Mathilde" uniqKey="Paul M" first="Mathilde" last="Paul">Mathilde Paul</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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